Friday, July 10, 2009

Getting busy now ><;

I've actually been given projects to work on >_<;; so I'm getting busy now... Though argh this morning... I must've ran up/down the stairs four times within an hour time because of the stupid Ventana machine.

So I started a run last night, or rather I delayed it to start at 7:30 this morning, and I was supposed to put the primer on at 9:00 but when I went up at 9:10 [yeah intense traffic this morning... I left the house at 8:20... It took us like 40mins to drive like ... 7miles...] to put the primer on and then I see that the Run Completion bar on top of the screen said 0% and that I was signed off. I was like wtf why'd that happen D: Maybe I made a mistake in the start time! So I went to get my supervisor. She came up and she's like wtf, so we started the run for the second time, skipping the deparafinization portion of the run because the tissues looked deparafinized after we opened the machine to check the slides.

I was supposed to put the primer on at 10:50, so at 10:40, I went up and 'lo and behold, the run completion was 0% AGAIN. I ran downstairs from the lab [since my lab and office are on different floors] and went to bother my superviser AGAIN. This time, we noticed a statement saying something about the EZPrep. My azn supervisor then asked my white supervisor what this was, and she was also like wtf. So my azn supervisor thought maybe the pressure was wrong or something with the EZPrep, so she took that out, and put it back in again. After, we started the run once again.

Guess what happened? So after like 13mins, the machine started beeping saying there was something wrong with the EZPrep. By now, my azn supervisor was like "okay this experiment is like fail bcause we've run it too many times, so now let's keep running it to try to get the machine to work."

This time, we filled the EZPrep more, since it was low [even though the machine said there was enough] and started the run AGAIN. We took out the deparafinization and the cell conditioning steps for this run. This time, I literally sat there for 35 mins watching the machine, making sure nothing happens again. So after it got past the EZPrep stage, I went downstairs to ask my supervisor what to do with the slides and she told me to just stop the run and throw them away, since they're probably bad by now anyways. She said we'll just have to rerun this next Wednesday, because my white supervisor needed to use it Monday and Tuesday.

Yeah so that was my morning until like 11:00am. After that... my azn supervisor gave me this huge list of ID numbers of something and input them into Spectrum database... and I've only finish one outta four pages so far because it's so freaking long and tedious. Arggggggggggg I wanna go home and sleep T_____T...

I doubt I'm going home anytime soon because my mom hasn't finished her work yet, but she has a volleyball tournament [company tournament]at 2pm [1 min from now]... then she needs to finish her huge load of work... [why does she even play if she has so much to do >_<;;;?]

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